Expressions of transcription factors in goldfish (Carassius auratus L.) macrophages and their progenitors.

نویسندگان

  • Barbara A Katzenback
  • Bahram V Foroutanpay
  • Miodrag Belosevic
چکیده

The development of macrophages is a highly regulated process requiring coordination amongst transcription factors. The presence/absence, relative levels, antagonism, or synergy of all transcription factors involved is critical to directing lineage cell fate and differentiation. While relative levels of many key myeloid transcription factors have been determined in mammalian macrophage differentiation, a similar set of studies have yet to be conducted in a teleost system. In this study, we report on the mRNA levels of transcription factors (cebpa, cjun, cmyb, egr1, gata1, gata2, gata3, lmo2, mafb, pax5, pu.1 and runx1) in sorted goldfish progenitor cells, monocytes, and macrophages from primary kidney macrophage cultures. The mRNA levels of runx1 and pu.1 were significantly higher, gata3 and pax5 mRNA levels were lower, in monocytes compared to progenitors, and the mRNA levels of cjun, egr1, gata2, gata3, mafb and pax5 were significantly decreased in macrophages compared to progenitor cells. The relative mRNA levels of the interferon regulatory factor family of transcription factors, irf1, irf2, irf5, irf7, irf8 and irf9 in sorted progenitors, monocytes and macrophages were also measured. In contrast to other irf family transcription factors examined, irf8 mRNA levels were increased in monocytes compared to progenitors by greater than three-fold, suggesting that irf8 is important for monopoiesis. Lastly, we show the differential regulation of myeloid transcription factor mRNA levels in sorted progenitor cells from 1, 2, or 3-day old cultures in response to the recombinant goldfish growth factors, rgCSF-1 and rgKITLA.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effects of Lactobacillus casei probiotics on growth performance, blood parameters and intestinal microbial flora in juvenile goldfish (Carassius auratus)

Lactobacillus casei probiotic is one of the probiotics used in aquaculture industry. The aim of this study was to evaluate the effect of Lactobacillus casei probiotic on the growth performance, blood parameters, and intestinal microbial flora in goldfish (Carassius auratus) for 60 days. In this study, 180 fish with an average weight of 10.11± 0.16 g were randomly distributed into two treatments...

متن کامل

Influence of salinity acclimatization on energy, oxygen consumption rate and glucose levels for Carassius auratus (Goldfish)

This study aimed to investigate the influence of increasing salinity concentrations on some indicators of energy consumption such as dissolved oxygen consumption, the consumed energy, as well as the levels of blood glucose in Carassius auratus (Goldfish), because of their direct relation with the metabolism rates when fish are exposed to exertion. Fish with an average weight of 37.5+2.5 g were ...

متن کامل

The effects of thyme oil extract to reduce toxic effects of Nanosilver on growth factors and serum biochemical in goldfish (Carassius auratus gibelio)

In this study, we divided 300 goldfish (average weight: 12.30 ± 2.11 g) into 4 groups. Fish were fed diets that contained 4 levels of thyme extract: 0 (control), 200, 400 and 800 mg/kg (Thyame, LN23IN, France) for a period of 2 weeks. At the end of 2 weeks, each group of the mentioned treatments was divided into 2 groups: nanosilver-exposed group and control. In the aquarium of the nanosilver-e...

متن کامل

Acute toxicity test of detergent powder in silver carp (Hypophthalmicthys molitrix), common carp (Cyprinus carpio), goldfish (Carassius auratus) and roach (Rutilus rutilus caspicus)

Different pollutants have undesirable effects on aquatic ecosystems, detergent powder is one of the elements in the sewages so study on the effects of this factor on  aquatic animals is crucial. The aim of the present study was to investigate acute effects of detergents as potentially dangerous additive to assess mortality effects of this product on some cultured fish. Silver Carp (Hypopht...

متن کامل

Effects of cortisol on tesicular apoptosis in goldfish (Carassius auratus)

Apoptosis is a programmed cell death that has been attributed to the action of stress hormones, such as cortisol. We investigated the in vitro effect of cortisol in goldfish (Carassius auratus) mature (GSI: 2.75-4.65) or immature (GSI: 1.94-2.4) testes. Testes were taken out and transferred into culture medium (M 199), cut into 20 mm thick slices and supplemented with fetal bovine serum (FBS), ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Developmental and comparative immunology

دوره 41 2  شماره 

صفحات  -

تاریخ انتشار 2013